|
KMID : 0364820090450020193
|
|
Korean Journal of Microbiology
2009 Volume.45 No. 2 p.193 ~ p.199
|
|
|
Isolation and Identification of Halotolerant Bacillus sp. SJ-10 and Characterization of Its Extracellular Protease
|
|
Kim Eun-Young
Kim Dong-Gyun
Kim Yu-Ri
Choi Sun-Young
Kong In-Soo
|
|
|
Abstract
|
|
|
|
A bacterium producing the halotolerant extracellular protease was isolated from squid jeotgal, and was identified as Bacillus sp. SJ-10 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The strain grew at 20oC~55oC, pH 5~8, and 0%~14% NaCl and optimal growth conditions were 35±5oC, pH 7, and 5% NaCl. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, and C16:0. DNA G+C content was 50.58 mol% and menaquinone consisted of MK-7. Phylogenic analysis based on the 16S rRNA gene sequence indicated that SJ-10T belongs to the genus Bacillus. About 40 kDa of the salt-tolerant protease was purified by 40% ammonium sulfate saturation and Mono Q column chromatography. The optimal activity of the protease was pH 8 and stable at pH 5~10. The optimum temperature and NaCl concentration were 35±5oC and 5±1%, respectively.
|
|
|
KEYWORD
|
|
|
Bacillus sp., collagen degrading enzyme, halophilic bacteria
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|